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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H | IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN194 |
| Description | Anti-EVI-5 Antibody |
| Alternate Names |
|
| Background Information | Also known as Ecotropic viral integration site 5 protein homolog, EVI-5 serves as a key regulator of the cell cycle through time-dependent stabilization of Emi1, FBX05 protein, and APC/C ubiquitination substrates, such as cyclin A. EVI-5 accumulates in early G1 following Plk1 destruction, but is degraded in a Plk1- and ubiquitin-dependent manner in early mitosis. EVI-5 ablation causes premature APC/C activation, SCF(betaTrCP)-dependent Emi1 degradation, and cyclin destruction, all of which leads to cell cycle arrest and cytokinesis failure. It exists as a monomer and a dimer, and it may form complexes with alpha- and gamma-tubulin or interact with the INCENP-aurora B kinase-survivin chromosomal passenger complex. EVI-5 is implicated in abnormal states, such as multiple sclerosis and neuroblastoma. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This EVI-5 antibody is validated for use in IHC & WB for the detection of the EVI-5 protein. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 2 µg/mL from a representative lot detected EVI-5 in NIH/3T3 nuclear extract. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the human EVI-5. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Mouse and Rat. Predicted to react with Human based upon immunogen design. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~95 kDa observed. Uncharacterized bands may be observed at ~31 kDa, ~17 kDa, and ~15 kDa in some cell lysates. This protein is known to have three potential phosphorylation sites. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in mouse and rat brain tissues. Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected EVI-5 in mouse and rat brain tissues. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |