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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H | ICC, IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN78 |
| Description | Anti-NeuN Antibody (rabbit) |
| Alternate Names |
|
| Background Information | RNA binding protein fox-1 homolog 3 (UniProt Q8BIF2; also known as Fox-1 homolog C, Fox-3, Hexaribonucleotide-binding protein 3, NeuN, Neuronal nuclei) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID 52897) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Originally characterized as a marker of post-mitotic neurons and named neuronal nuclei (NeuN), Rbfox3 plays a role in promoting neuronal differentiation through alternative splicing of Numb pre-mRNA during brain development. Rbfox3-knockout in mice is reported to result in defective hippocampal gene expression, defects in synaptic transmission and plasticity in the dentate gyrus, as well as increased seizure susceptibility and decreased anxiety-related behaviors. Likewise, human RBFOX3 gene mutations have been linked to neurodevelopmental delay, cognitive impairments, autistic features, and epilepsy. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NeuN (rabbit polyclonal), Cat. No. ABN78, is rabbit polyclonal antibody that detect NeuN and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected NeuN in Mouse Brain Nuclear Extract. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected NeuN in human cerebral cortex and mouse hippocampus tissue sections. Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in E18 neuronal cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to first 97 amino acids from the N-terminal region of murine NeuN. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects NeuN. It targets an epitope within first 97 amino acids from the N-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Snail based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~48/42 kDa observed; 40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Mouse E16 Brain lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected NeuN in Mouse E16 Brain lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |