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Millipore Milli-Mark Anti-Phosphotyrosine-Pe Antibody, Recombinant Clone 4g10 Is An Antibody Against Phosphotyrosine Clone 4g10 For Use In Fc. - Mill (Additional S&H or Hazmat Fees May Apply)
Some of the tyrosine residues can be tagged with a phosphate group by protein kinases and in its phosphorylated state, it is referred to as phosphotyrosine. Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phosphotyrosine antibodies is one of the significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosine phosphorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyne phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Product Information
Format
Phycoerythrin
Control
Treated versus untreated A431 cells
Presentation
Purified mouse monoclonal IgG2bk conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality Level
MQ100
Applications
Application
Milli-Mark Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10 is an antibody against Phosphotyrosine clone 4G10 for use in FC.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Phosphotyramine-KLH
Clone
4G10
Host
Mouse
Specificity
Antibody recognizes tyrosine-phosphorylated proteins from all species
Isotype
IgG2bκ
Species Reactivity
Vertebrates
Species Reactivity Note
Tested and passed on Human. Expected to detect tyrosyne phosphorylated proteins in all species.
Antibody Type
Monoclonal Antibody
Purification Method
Protein G Purified
Molecular Weight
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Product Usage Statements
Quality Assurance
Evaluated by flow cytometry using EGF treated versus untreated A431 cells
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
