Key Specifications Table
| Source | Purity |
|---|---|
| Protein is expressed in E.coli. The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided. | Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Description | |
|---|---|
| Catalogue Number | GF321 |
| Description | FGF-acidic, Human Recombinant Animal Free |
| Alternate Names |
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| Background Information | FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGFacidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGFacidic is a 16.0 kDa protein consisting of 141 amino acid residues. |
| Product Information | |
|---|---|
| Presentation | Product is presented in 5 mM Sodium Phosphate, pH 8.0 + 100mM NaCl. and is filtered through a 0.2 micron filter before lyophilization. |
| Quality Level | MQ100 |
| Biological Information | |
|---|---|
| Purity | Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Source | Protein is expressed in E.coli. The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided. |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Bioactivity assay: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/ml corresponding to a specific activity of ≥ 1 x 10^5 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.5 ng/ml in the presence of 10 μg/ml heparin, corresponding to a specific activity of ≥ 2 x 10^6 units/mg. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate, pH 8.0 to a concentration of < 0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 50µg |