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Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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H | WB, IH(P) | M | Purified | Monoclonal Antibody |
Description | |
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Catalogue Number | MABE337 |
Description | Anti-Hexim 1 Antibody, clone 10D2.1 |
Alternate Names |
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Background Information | Hexim1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. Hexim1 may also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), Hexim1, Hexim2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. Hexim1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. Hexim1 is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen. |
Product Information | |
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Format | Purified |
Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
Quality Level | MQ100 |
Applications | |
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Application | This Anti-Hexim 1 Antibody, clone 10D2.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Hexim 1. |
Key Applications |
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Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Hexim 1 in human placenta tissue. |
Biological Information | |
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Immunogen | GST-tagged recombinant protein corresponding to the C-terminus of human Hexim 1. |
Epitope | C-terminus |
Clone | 10D2.1 |
Concentration | Please refer to lot specific datasheet. |
Host | Mouse |
Isotype | IgG2bκ |
Species Reactivity |
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Antibody Type | Monoclonal Antibody |
Entrez Gene Number |
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Gene Symbol |
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Purification Method | Protein G Purified |
UniProt Number |
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Molecular Weight | ~60 kDa observed |
Product Usage Statements | |
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Quality Assurance | Evaluated by Western Blotting in human placenta tissue lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected Hexim 1 in 10 µg of human placenta tissue lysate. |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
Packaging Information | |
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Material Size | 200 µg |