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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF297 |
| Description | Anti-RIG-I, clone 1C3 Antibody |
| Alternate Names |
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| Background Information | Retinoic acid-inducible gene 1 protein (RIG-1) is a cytoplasmic DExD/H box RNA helicase belonging to the helicase family and containing 2 CARD domains within the N-terminus, a repressor domain (RD), a helicase ATP-binding domain and a helicase C-terminal binding domain. RIG-1 is able to bind the 5’-triphosphate RNA of single or double-stranded viral RNA. After recognizing and binding viral RNA, RIG-1 protein initializes type I IFN production, and within fibroblasts and dendritic cells plays a central role in the regulation of type I IFN production in response to viral presence. There are four known isoforms resulting from alternative splice variants. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-RIG-I, clone 1C3 Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of RIG-I. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot immunoprecipitated RIG-1 in A-549 infected with SeV cell lysate (Baum, A., et al. (2011). PNAS. 108(7):16303-16308). Immunoprecipitation Analysis: A representative lot immunoprecipitated RIG-1 in A-459 lung carcinoma cell lysate (Nistal-Villan, E., et al. (2010). J. Biol. Chem. 285:20252-20261). Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected RIG-I in HeLa cells (Courtesy of Maite Sanchez). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human RIG-I. |
| Clone | 1C3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1λ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~110 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A-549 infected with SeV cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected RIG-1 in 10 µg of A-549 infected with SeV cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |