We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Share this :
Print
Genscript CD40L/CD154/TRAP, Human
Genscript CD40L/CD154/TRAP, Human - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$1,200.00
Your Price
$1,200.00
HOW MUCH YOU SAVE:
0.00 %
NETA PART: | GSCRPT-Z02022-1 |
MFG.PART: | Z02022-1 |
UNSPSC: | 41105300 |
Manufacturer: | GenScript USA Inc |
Product Introduction
Species | Human | |
Protein Construction |
| |
Purity | > 95% as analyzed by SDS-PAGE > 95% as analyzed by RP-HPLC > 95% as analyzed by Anion-exchange FPLC | |
Endotoxin Level | < 1 EU/μg of protein by LAL method | |
Biological Activity | Recombinant human CD40 Ligand (CD40L/CD154/TRAP) is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5.0-10.0 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg. | |
Expression System | E. coli | |
Theoretical Molecular Weight | 16.3 kDa | |
N-terminal Sequence Analysis | The sequence of the first five N-terminal amino acids was determined and found to be Met-Gln-Lys-Gly-Asp. | |
Formulation | Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0, 250 mM NaCl buffer. | |
Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile 18 MΩ-cm H₂O up to 100 µg/ml. | |
Storage & Stability | Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
Background
Target Background | CD40 Ligand (CD40L/CD154/TRAP) is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-γ, TNF-α, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. |
Synonyms | CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154; CD40LG; TNFSF5 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
SKU | GSCRPT-Z02022-1 |
---|---|
Supplier Part Number | Z02022-1 |
UM | EA |
UNSPSC | 41105300 |
Manufacturer | GenScript USA Inc |
ProductLine | GSCRPT |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 7 Business Days |