Takara pTet-On Advanced Vector \20 ug

Takara The pTet-On Advanced Vector is used to create stable Tet-On Advanced cell lines as hosts for a Tet-inducible expression system. The Tet-On Advanced protein is a modified Tet-responsive reverse transactivator (rtTA-Advanced) that binds tightly to tetracycline-responsive elements (TRE) and strongly activates transcription of a downstream gene in the presence of doxycycline (Dox; a Tc derivative). As Dox is added to the culture medium, transcription from the TRE or TRE-Tight is turned on in a highly sensitive and dose-dependent manner.The Tet-On Advanced gene is fully synthetic and has been optimized for expression and stability in mammalian cells by incorporating mammalian codon preferences and by removing cryptic splice sites from the mRNA. In addition, 3 minimal "F-type" HSV VP16 transcription activation domains provide maximal activation and reduced toxicity. The immediate early promoter of cytomegalovirus (PCMV IE) produces strong constitutive expression of Tet-On Advanced, which accumulates to high levels. Stable Tet-On Advanced cell lines can be generated by transfecting the plasmid and selecting for G418-resistant clones.- TAKBGN (Additional S&H or Hazmat Fees May Apply)