Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications |
|---|---|
| H | WB, IP, RIP |
| Description | |
|---|---|
| Catalogue Number | 03-116 |
| Trade Name |
|
| Description | RIPAb+™ STAU1 (Staufen 1) - RIP Validated Antibody and Primer Set |
| Overview | RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation. STAU1 (Staufen 1) is a RNA-binding protein implicated in the transport and/or localization of mRNA via the microtubule network to the rough endoplasmic reticulum. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Anti-STAU1 (Staufen 1) (Rabbit Polyclonal),. Lyophilized powder. Add 50 μL of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. Normal Rabbit IgG. One vial containing 125 µg of purified rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C. RIP Primers, ARF1. One vial containing 75 μL of 5 μM of each primer specific for human ARF1 mRNA. Store at -20°C. FOR: GAC CAC GAT CCT CTA CAA GC REV: TCC CAC ACA GTG AAG CTG ATG |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This RIPAb+ STAU1 (Staufen 1) -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. |
| Key Applications |
|
| Application Notes | Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or 5 µg of Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405). Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: STAU1) were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232) . Arrow indicates STAU1. (Figure 2). Western Blot Analysis: Representative lot data. HepG2 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-STAU1 antibody (Cat. # CS204405, 1:1000 dilution). Proteins were visualized using a Goat Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system. Arrow indicates STAU1 (~63 kDa). (Figure 3). Note: Uncharacterized bands appear at ~22 kDa and ~53 kDa in |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from amino acids 2-51 of human STAU 1. |
| Epitope | a.a. 2-51 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human. Other species have not yet been tested. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Molecular Weight | ~63 kDa observed. Uncharacterized bands appear at ~22 kDa and ~53 kDa in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal Rabbit IgGor 5 µg of Anti-STAU1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of STAU1-associated RNA was verified by qPCR using RIP Primers ARF1, (Figure 1). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 10 assays |
| Material Package | 10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context). |