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Millipore Anti-P47-Phox Antibody Detects Level Of P47-Phox & Has Been Published & Validated For Use In Ic, Ip & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%
Quality Level
MQ100
Applications
Application
Anti-p47-phox Antibody detects level of p47-phox & has been published & validated for use in IC, IP & WB.
Key Applications
Immunocytochemistry
Immunoprecipitation
Western Blotting
Biological Information
Immunogen
GST fusion protein corresponding to full-length human p47-phox
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
Recognizes p47 phox.
Isotype
IgG
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_000265
Entrez Gene Summary
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
Gene Symbol
NCF1
NCF-47K
NOXO2
p47-phox
SH3PXD1A
NCF1A
p47phox
NCF-1
Purification Method
Unpurified
UniProt Number
P14598
UniProt Summary
FUNCTION: SwissProt: P14598 # NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). SIZE: 390 amino acids; 44683 Da SUBCELLULAR LOCATION: Cytoplasm. DISEASE:SwissProt: P14598 # Defects in NCF1 are the cause of autosomal cytochrome-b- positive chronic granulomatous disease type 1 (CGD) [MIM:233700]; an autosomal recessive form. SIMILARITY: SwissProt: P14598 ## Contains 1 PX (phox homology) domain. & Contains 2 SH3 domains.
Molecular Weight
47 kDa
Product Usage Statements
Quality Assurance
routinely evaluated by immunoblot on RIPA lysates from HL-60 cells
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
