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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | IP, WB, ICC | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-122 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Kalirin Antibody |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide prior to the addition of glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Kalirin Antibody is an antibody against Kalirin for use in IP, WB & IC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein corresponding to residues 517-976 of rat Kalirin, cleaved with thrombin to remove the GST moiety; referred to as JH2582 |
| Host | Rabbit |
| Specificity | Kalirin; does not cross react with Trio |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Huntingtons disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1, which is a huntingtin binding protein that may function in vesicle trafficking. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Gene Symbol |
|
| Purification Method | Protein A chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O60229 # Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity. COFACTOR: Magnesium. SIZE: 2985 amino acids; 340174 Da SUBUNIT: Interacts with the C-terminal of peptidylglycine alpha- amidating monooxygenase (PAM) and with the huntingtin-associated protein 1 (HAP1) (By similarity). SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Note=Associated with the cytoskeleton. TISSUE SPECIFICITY: Isoform 2 is brain specific. Highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus. Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle. DOMAIN: SwissProt: O60229 The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity (By similarity). PTM: Autophosphorylated. DISEASE: SwissProt: O60229 # Genetic variation in KALRN is associated with susceptibility to coronary heart disease type 5 (CHDS5) [MIM:608901]. CHD is the leading cause of death and disability worldwide. CHD is multifactorial disease with a strong genetic component. Classic epidemiologic studies have revealed many risk factors for CHD, including age, sex, hypertension, dyslipidemia, diabetes mellitus, smoking, and physical inactivity. SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 CRAL-TRIO domain. & Contains 2 DH (DBL-homology) domains. & Contains 1 fibronectin type-III domain. & Contains 1 Ig-like C2-type (immunoglobulin-like) domain. & Contains 2 PH domains. & Contains 1 protein kinase domain. & Contains 1 RPR domain. & Contains 2 SH3 domains. & Contains 5 spectrin repeats. MISCELLANEOUS: Called DUO because the encoded protein is closely related to but shorter than TRIO. |
| Molecular Weight | 190, 115, 370 and 420kDa isoforms |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from rat brain microsomal protein preparation; long exposure times may be required |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |