| Description | |
|---|---|
| Catalogue Number | 14-516 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Cdk5/p25 Protein, active, 10 µg |
| Overview | Complex of N-terminal His6-tagged, recombinant, full-length human cdk5 and Nterminal GST-tagged, recombinant, full-length human p25 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active complex of N-terminal His6-tagged, recombinant, full-length human Cdk5 & N-terminal GST-tagged, recombinant, full-length human p25, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Co-expressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Gene Symbol |
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| Protein Target | CDK5/p25 |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | CMGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q00535 # Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase. SIZE: 292 amino acids; 33304 Da SUBUNIT: Heterodimer of a catalytic subunit and a regulatory subunit (p35). Found in a trimolecular complex with CABLES1 and ABL1. Interacts with CABLES1 (By similarity). Interacts with AATK. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, growth cone (By similarity). Note=In axonal growth cone with extension to the peripheral lamellipodia (By similarity). SIMILARITY: SwissProt: Q00535 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 34.4kDa (cdk5), 49.4kDa (p25) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of Histone H1 |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-516M when ordering the 250 µg size. |