| Description | |
|---|---|
| Catalogue Number | 14-603 |
| Brand Family | Upstate |
| Trade Name |
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| Description | PI3 Kinase (p110β/p85α) Protein, active, 20 µg |
| Overview | Complex of N-terminal His6-tagged recombinant full-length human p110β and untagged, recombinant, full length, human p85&alpha |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active complex of N-terminal His6-tagged recombinant full-length human p110β & untagged, recombinant, full length, human p85&alpha. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Coexpressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
|
| Entrez Gene Summary | Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM] |
| Gene Symbol |
|
| Protein Target | PI3Kβ/p85α |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | PI3K/PI4K |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P42338 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. SIZE: 1070 amino acids; 122762 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. TISSUE SPECIFICITY: Expressed ubiquitously. SIMILARITY: SwissProt: P42338 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain. |
| Molecular Weight | p110β (124kDa) & p85&alpha (83.7kDa) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated in a 30 minute reaction using the PIProfiler™ protocol (see www.upstate.com/features/piprofiler). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 20 µg |
| Material Package | Also available in 250μg size as catalog # 14-603M - Call for pricing and availability. |