Description | |
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Catalogue Number | 14-656 |
Brand Family | Upstate |
Trade Name |
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Description | LIM Kinase 1 Protein, active, 10 µg |
Overview | N-terminal 6His-tagged, recombinant, human LIM Kinase 1, amino acids 285-638 |
Product Information | |
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Quality Level | MQ100 |
Applications | |
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Application | Active, N-terminal 6His-tagged, recombinant, human LIM Kinase 1, amino acids 285-638, for use in Kinase Assays. |
Key Applications |
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Biological Information | |
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Source | expressed by baculovirus in Sf21 cells |
Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
Entrez Gene Number |
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Entrez Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. |
Gene Symbol |
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Protein Target | LIMK1 |
Purification Method | Ni2+/NTA-agarose |
Target Sub-Family | TKL |
UniProt Number |
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UniProt Summary | FUNCTION: SwissProt: P53667 # Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development. SIZE: 647 amino acids; 72585 Da SUBUNIT: Self-associates. The LIM domain interacts with the cytoplasmic domain of NRG1. Binds ROCK1. Interacts with SSH1. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. PTM: Autophosphorylated (By similarity). & Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1. DISEASE: SwissProt: P53667 # Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23. SIMILARITY: SwissProt: P53667 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 2 LIM zinc-binding domains. & Contains 1 PDZ (DHR) domain. & Contains 1 protein kinase domain. |
Molecular Weight | 41.2kDa |
Product Usage Statements | |
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Quality Assurance | routinely evaluated by phosphorylation of Cofilin 1 (Catalog# 12-556) |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Stable for 6 months at -70°C from date of shipment |
Packaging Information | |
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Material Size | 10 µg |