| Description | |
|---|---|
| Catalogue Number | 14-660 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Itk Protein, active, 10 µg |
| Overview | N-terminal 6His-tagged, recombinant, human Itk, amino acids 352-617. |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal 6His-tagged, recombinant, human Itk, amino acids 352-617. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Human Itk expressed by baculovirus in Sf21 cells. |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. |
| Gene Symbol |
|
| Protein Target | Itk |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | TK |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q08881 # Plays a role in T-cell proliferation and differentiation. COFACTOR: Binds 1 zinc ion per subunit (By similarity). SIZE: 620 amino acids; 71831 Da SUBCELLULAR LOCATION: Cell membrane (By similarity). Note=Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T- cells (By similarity). TISSUE SPECIFICITY: T-cell lines and natural killer cell lines. SIMILARITY: SwissProt: Q08881 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. & Contains 1 Btk-type zinc finger. & Contains 1 PH domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain. |
| Molecular Weight | 34kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of MBP. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | 2 vials at 5μg each |