| Description | |
|---|---|
| Catalogue Number | 14-918 |
| Trade Name |
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| Description | JAK1 Protein, active, 10 µg |
| Overview | Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. |
| Product Information | |
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| Presentation | 50mM Tris/HCl pH7.5, 150mM NaCl, 5mM β-glycerophosphate, 0.5mM sodium orthovanadate, 50% v/v glycerol, 0.02% Triton® X-100, 0.5mM EDTA, 2mM DTT. Frozen solution. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal GST-tagged, recombinant, human JAK1, amino acids 866-end, expressed by baculovirus in Sf21 insect cells. Activated in vitro by auto-phosphorylation. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
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| Source | N-terminal GST-tagged, recombinant, human JAK1 amino acids 866-end, expressed by baculovirus in Sf21 insect cells. Activated in vitro by auto-phosphorylation. |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Purified using glutathione sepharose. |
| Target Sub-Family | TK |
| UniProt Number |
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| Molecular Weight | 61 kDa |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated by phosphorylation of peptide GEEPLYWSFPAKKK. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
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| Material Size | 10 µg |
| Material Package | Also available in 250μg size as catalog # 14-918M and in bulk as catalog # 14-918-K. |