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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1847 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified IgG in PBS containing 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop detects level of C-X-C Chemokine Receptor 4 & has been published & validated for use in WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:500 - 1000 Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Extracellular loop peptide of human CXCR4 (Berson et al. 1996; Leotscher et al. 1994). |
| Epitope | extracellular loop |
| Host | Rabbit |
| Specificity | Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (Nimitrov 1997). Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principle coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (Feng et al. 1996; Berson et al. 1996). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (Doranz et al. 1996; Endres et al. 1996). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (Bleul et al. 1996; Oberlin et al. 1996). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (Lapham et al. 1996). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (Feng et al. 1996). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (Feng et al. 1996; Endres et al. 1996; Brelot et al. 1997). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites (Brelot et al. 1997; Lu et al. 1997). |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P61073 # Receptor for the C-X-C chemokine CXCL12/SDF-1. Transduces a signal by increasing the intracellular calcium ions level. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. SIZE: 352 amino acids; 39746 Da SUBUNIT: Interacts with HIV-1 surface protein gp120 and Tat. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. DOMAIN: SwissProt: P61073 The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity. PTM: Sulfated. DISEASE: SwissProt: P61073 # Defects in CXCR4 are a cause of WHIM syndrome [MIM:193670]; also called warts, hypogammaglobulinemia, infections, and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis. SIMILARITY: Belongs to the G-protein coupled receptor 1 family. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Can be refrigerated at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |