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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB4254 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Proteinase-Activated Receptor 2 Antibody, NT, extracellular |
| Alternate Names |
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| Background Information | The Proteinase-activated receptor 2 (PAR2) is a member of the proteinase-activated receptor subfamily. It is activated through proteolytic exposure of an occult tethered ligand by trypsin and trypsin-like proteases. This is in contrast to other members of the subfamily which are activated by the protease thrombin. PAR2 has been implicated in acute inflammatory response, asthma, and pain transmission. PAR2 expression has been documented in the periphery. ESTs have been isolated from adrenal, brain, breast, heart/melanocyte/uterus, kidney, lung, and vessel libraries. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Peptide-affinity purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.7, containing 0.01% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Proteinase-Activated Receptor 2 Antibody, N-terminal, extracellular is validated for use in IH(P) for the detection of Proteinase-Activated Receptor 2. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (formalin-fixed, paraffin-embedded tissues) Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to a sequence within the N-terminal extracellular domain of human PAR2. The immunogen has 70% homology with mouse and 60% homology with rat. |
| Epitope | N-terminal, extracellular |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes human PAR2. The specificity of this antibody has been confirmed by IHC staining of human pancreas. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Coagulation factor II (thrombin) receptor-like 1 (F2RL1) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. The F2RL1 gene contains two exons and is widely expressed in human tissues. The predicted protein sequence is 83% identical to the mouse receptor sequence. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P55085 # Receptor for trypsin and trypsin-like enzymes coupled to G proteins that stimulate phosphoinositide hydrolysis. May have a role in the regulation of vascular tone. SIZE: 397 amino acids; 44126 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed in tissues with especially high levels in pancreas, liver, kidney, small intestine, and colon. Moderate expression is detected in many organs, but none in brain or skeletal muscle. PTM: A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. SIMILARITY: SwissProt: P55085 ## Belongs to the G-protein coupled receptor 1 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |