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Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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H, M, R | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
Description | |
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Catalogue Number | ABN182 |
Description | Anti-CYP27B1 Antibody |
Alternate Names |
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Background Information | 25-hydroxyvitamin D-1 alpha hydroxylase (1αOHase; VD3 1A hydroxylase; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; or CYP27B1) is a mitochondrial enzyme that belongs to the cytochrome P450 family. It catalyzes the hydroxylation of 25-hydroxyvitamin D3 to form an active 1-alpha, 25-dihydroxyvitamin D3, in the kidneys and other tissues. 25-hydroxyvitamin D-1 alpha hydroxylase plays an important role in bone growth, calcium metabolism, and tissue differentiation. Previous studies have reported that mice deficient in 25-hydroxyvitamin D-1 alpha hydroxylase are affected by hypocalcemia, secondary hyperparathyroidism, retarded growth, and the skeletal abnormalities. In humans, defects in this enzyme causes pseudovitamin D deficiency rickets (vitamin D-dependent type 1A rickets), an autosomal recessive condition characterized by abnormal bone mineralization and impaired growth. |
Product Information | |
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Format | Affinity Purified |
Control |
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Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
Quality Level | MQ100 |
Applications | |
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Application | This Anti-CYP27B1 Antibody is validated for use in Western Blotting, IHC for the detection of CYP27B1. |
Key Applications |
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Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CYP27B1 in mouse and rat hippocampus tissue and in rat kidney tissue. |
Biological Information | |
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Immunogen | KLH-conjugated linear peptide corresponding to human CYP27B1. |
Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
Host | Rabbit |
Species Reactivity |
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Species Reactivity Note | Demonstrated to react with Human, Mouse, and Rat. Predicted to react with Chimpanzee based on 100% sequence homology. |
Antibody Type | Polyclonal Antibody |
Entrez Gene Number |
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Gene Symbol |
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Purification Method | Affinity Purfied |
UniProt Number |
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Molecular Weight | ~58 kDa observed. Uncharacterized bands may be observed at ~55 kDa, ~70 kDa, ~110 kDa, and ~115 kDa in some cell lysates. A doublet at ~58 kDa may be observed in some cell lysates (Kemmis, C. M., et al. (2006). J Nutr. 136(4):887-892.). |
Product Usage Statements | |
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Quality Assurance | Evaluated by Western Blot in serum free MCF-7 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected CYP27B1 in 10 µg of serum free MCF-7 cell lysate. |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
Packaging Information | |
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Material Size | 100 µg |