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Key Specifications Table
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Enzyme Assays |
Description | |
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Catalogue Number | ECM675 |
Brand Family | Chemicon® |
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Description | 3D Collagen Culture Kit |
Overview | Cell migration is a fundamental function of normal cellular processes, including cell proliferation, and migration. Cell behavior in vitro is typically examined in a 2D environment; however, in the body, cells exist in a three-dimensional extracellular matrix environment rich in type I collagen. In fact, cells cultured in 3D matrices better reflect in vivo cell physiology when compared to traditional 2D systems. CHEMICON (EMD Millipore) has developed a three-dimensional cell culture system that closely simulates the collagen-rich extracellular matrix of normal tissue. This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents. As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis. For Research Use Only; Not for use in diagnostic procedures |
Materials Required but Not Delivered | · Tissue Culture Dishes · Pipettes and Sterile Tips · Microcentrifuge Tubes, Sterile · 37oC Incubator · Inverted Light Microscope · Bacterial Collagenase |
Product Information | |
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Components |
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Presentation | Collagen solution is supplied in 6mM acetate buffer at ~ pH7.0. |
Quality Level | MQ100 |
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Key Applications |
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Application Notes | This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents. As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis. |
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Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Store kit materials at 4°C up to their expiration date. |
Packaging Information | |
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Material Size | 1 kit |