Key Specifications Table
| Source | Purity |
|---|---|
| Hela Cells | Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 95% pure by SDS-PAGE gel and HPLC analyses |
| Description | |
|---|---|
| Catalogue Number | GF164 |
| Description | ANG-1 Protein, Human Recombinant |
| Alternate Names |
|
| Background Information | Angiopoietin1 (Ang1) and Angiopoietin2 (Ang2) are two closely related secreted ligands which bind with similar affinity to Tie2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1/Tie-2 signaling appears to be regulated by Angiopoietin-2 (Ang-2), a natural antagonist for Tie-2 that exerts its effects through an internal autocrine loop mechanism. In addition to suppressing endothelial cell activation by inhibiting the expression of adhesion and inflammatory molecules, Ang-1 enhances endothelial cell survival and capillary morphogenesis, and lessens capillary permeability. As such, Ang-1 has a potential to become an effective therapeutic agent for treating various endothelium disorders, including several severe human pulmonary diseases. Recombinant human ANG-1, derived from HeLa cells, is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 – 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein. |
| Product Information | |
|---|---|
| Presentation | Product is filtered through a 0.2 micron filter before lyophilization. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | The Ang-1 protein & Ang-2 protein are secreted ligands which bind with similar affinity to Tie2, a receptor tyrosine kinase with immunoglobulin & epidermal growth factor homology domains expressed on endothelial & early hematopoietic cells. |
| Biological Information | |
|---|---|
| Purity | Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 95% pure by SDS-PAGE gel and HPLC analyses |
| Source | Hela Cells |
| Cross Reactivity | None |
| UniProt Number |
|
| Product Usage Statements | |
|---|---|
| Quality Assurance | Bioactivity assay: Determined by its ability to induce adhesion of human umbilical vein endothelial cells (HUVEC). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20°C for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 20 µg |