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Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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Ch, H | FC, IHC, FUNC | M | Purified | Monoclonal Antibody |
Description | |
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Catalogue Number | MAB1961Z |
Brand Family | Chemicon® |
Trade Name |
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Description | Anti-Integrin αVβ5 Antibody, clone P1F6, azide free |
Alternate Names |
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Background Information | The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha V chain. Alpha chain V undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. |
Product Information | |
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Format | Purified |
Control |
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Presentation | Purified immunoglobulin in 0.01M phosphate buffer, pH 7.1, 0.15M NaCl containing no preservatives. |
Quality Level | MQ100 |
Applications | |
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Application | Detect Integrin αVβ5 using this Anti-Integrin αVβ5 Antibody, clone P1F6, azide free validated for use in FC, IH. |
Key Applications |
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Applications Not Recommended |
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Application Notes | Inhibition of attachment of HT29 cells to vitronectin: >1:1,000 Inhibition of attachment of carcinoma and melanoma cells to vitronectin Immunohistochemistry: for use on acetone or paraformaldehyde fixed tissue. (Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.) Immunocytochemistry: for use on human carcinomas, normal epithelium and keratinocytes 1:1500 (Acetone Fixed Only) Immunoprecipitation FACS EIA Optimal working dilutions must be determined by the end user. |
Biological Information | |
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Immunogen | Human UCLA P3 cells (lung carcinoma) |
Clone | P1F6 |
Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
Host | Mouse |
Specificity | Reacts with the human integrin alphaVbeta5. |
Isotype | IgG1 |
Species Reactivity |
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Antibody Type | Monoclonal Antibody |
Entrez Gene Number |
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Entrez Gene Summary | ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. |
Gene Symbol |
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Purification Method | Protein A Purfied |
UniProt Number |
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UniProt Summary | FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions. SIZE: 1048 amino acids; 116038 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. |
Product Usage Statements | |
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Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
Packaging Information | |
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Material Size | 100 µg |