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Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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H | ELISA, FC, IHC, IP, FUNC, WB | M | Purified | Monoclonal Antibody |
Description | |
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Catalogue Number | MAB1973Z |
Brand Family | Chemicon® |
Trade Name |
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Description | Anti-Integrin α1 Antibody, clone FB12, azide free |
Alternate Names |
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Product Information | |
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Format | Purified |
Control |
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Presentation | Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl. |
Quality Level | MQ100 |
Applications | |
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Application | Detect Integrin α1 using this Anti-Integrin α1 Antibody, clone FB12, azide free validated for use in ELISA, FC, IH, IP, FUNC & WB. |
Key Applications |
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Application Notes | Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation. Immunoblotting: 2 μg/mL Immunoprecipitation: 1 μg/mL FACS analysis: 2 μg/mL ELISA: 0.5 μg/mL Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri, 1996): 1-5 μg/mL Optimal working dilutions must be determined by end user. |
Biological Information | |
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Clone | FB12 |
Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
Host | Mouse |
Specificity | Reacts with the I domain (Val151 - Ala364) of human alpha1 integrin (Fabbri, 1996). Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi, 1991). Does not recognize recombinant I domain of rat integrin alpha1. |
Isotype | IgG1 |
Species Reactivity |
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Antibody Type | Monoclonal Antibody |
Entrez Gene Number |
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Entrez Gene Summary | This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. |
Gene Symbol |
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Purification Method | Protein A Purfied |
UniProt Number |
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UniProt Summary | FUNCTION: SwissProt: P56199 # Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen. SIZE: 1179 amino acids; 130848 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: SwissProt: P56199 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain. |
Molecular Weight | 130 kDa |
Product Usage Statements | |
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Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
Packaging Information | |
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Material Size | 100 µg |