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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB5364 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Reelin Antibody, a.a. 164-496 mreelin, clone G10 |
| Background Information | The highly layered structure of the cerebral cortex is established through the pattern of neuronal cell migrations. The first step is the creation of the primordial layer, the preplate, consisting of radial glial cells and the earliest generated neurons. Among these neurons are the Cajal-Retzius neurons. In the next step, the preplate splits into a superficial (marginal) zone, where the Cajal-Retzius neurons reside, and a deep subplate wherein the neurons form. Neurons migrating from the subplate form the cortical plate. This migration takes place on the radial glial fibers. The reeler mutant in mouse displays an abnormal pattern of cell migration throughout the cerebral and cerebellar cortices. The preplate forms normally, and the neurons differentiate at the correct times in the ventricular zone. However, instead of forming the normal "inside-out" arrangement of neurons in the cortical plate, the older neurons are found furthest from the ventricular zone, while the younger neurons do not migrate far at all. The reeler cerebral cortex is inverted from that of the wild type mouse. The defect of the reeler mice appears to be in the production of an extracellular matrix protein by the Cajal-Retzius cells (DArcangelo et al., 1995, Nature 374:719-723.; Ogawa et al., 1995 Neuron 14:899-912.) This 388kDa protein is made by wild-type mice but not by the reeler mutants. It is thought that this Reelin protein is crucial for positioning the migrating neuron within the cortical plate (Figure 1). In the absence of Reelin, the migrating neuron would be "lost," and the cortical plate would be abnormal. We do not yet know the mechanisms by which Reelin informs the cells as to their position, how the cell responds to Reelin, and why the absence of reelin should give an "inverted" plate. However, the identification of the protein encoded by the reeler gene should allow us to begin these studies. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer with 0.25 M NaCl and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Reelin using this Anti-Reelin Antibody, a.a. 164-496 mreelin, clone G10 validated for use in IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A previous lot of this antibody was used in IH. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant reelin amino acids 164-496 |
| Epitope | a.a. 164-496 mreelin |
| Clone | G10 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reelin |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, rat and rodent. The antibody shows weak reactivity to reelin from other species. Expected to react with human based on sequence homolog. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P78509 # Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and ApoER2 induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (By similarity). SIZE: 3460 amino acids; 388402 Da SUBUNIT: Binds to the ectodomains of VLDLR and ApoER2 (By similarity). SUBCELLULAR LOCATION: Secreted (By similarity). TISSUE SPECIFICITY: Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Also expressed in fetal and adult liver.DEVELOPMENTAL STAGE: Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum. DOMAIN: SwissProt: P78509 The basic C-terminal region is essential for secretion (By similarity). DISEASE: SwissProt: P78509 # Defects in RELN are the cause of autosomal recessive lissencephaly with cerebellar hypoplasia [MIM:257320]; also known as Norman-Roberts syndrome. Some patients also displayed persistent lymphedema neonatally, and one showed accumulation of chlyous or fatty, ascites fluid. & Defects in RELN may contribute to susceptibility to schizophrenia. Expression of the protein is reduced to about 50% in patients with schizophrenia. & Defects in RELN may predispose to autistic disorder. A polymorphic GGC triplet repeat located in the 5-UTR region of RELN gene, which harbors in the normal population 8 to 10 repeats, is significantly increased in autistic patients to carry 4 to 23 additional repeats. SIMILARITY: Belongs to the reelin family. & Contains 15 BNR repeats. & Contains 8 EGF-like domains. & Contains 1 reelin domain. |
| Molecular Weight | ~388 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on Rat brain lysates. Western Blotting Analysis: 1:500 dilution of this antibody detected reelin on 10 μg of Rat brain lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |