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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ELISA, EMSA, IP, WB | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB5478 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Retinoid X Receptor α Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites fluid. Liquid. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Retinoid X Receptor α Antibody (Mouse Monoclonal Antibody) validated in ELISA, EMSA, IP, WB to detect Retinoid X Receptor α also known as RXR alpha. |
| Key Applications |
|
| Application Notes | Western blot (endogenous and recombinant protein): 1:500-1:5,000 Immunocytochemistry (transfected cells): 1:500-1:5,000 Immunoprecipitation (endogenous and recombinant protein): 1:500-1:5,000 Gel shift (endogenous and recombinant protein): 1:500-1:5,000 ELISA: 1:500-1:5,000 Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Fusion protein corresponding to the DE-region of mouse RXRa. |
| Host | Mouse |
| Specificity | Reacts with Retinoid X Receptor alpha (RXRalpha). No cross reactivity with RXRbeta or RXRgamma. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P19793 # Nuclear hormone receptor. Involved in the retinoic acid response pathway. Binds 9-cis retinoic acid (9C-RA). ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer (By similarity). SIZE: 462 amino acids; 50811 Da SUBUNIT: Homodimer or forms a heterodimer with peroxisome proliferator activated receptor gamma called adipocyte-specific transcription factor ARF6. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes (By similarity). Interacts with SFPQ. Interacts with HCV core protein. Interacts with PELP1. Interacts with SENP6. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Highly expressed in liver, also found in lung, kidney and heart. DOMAIN: SwissProt: P19793 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. PTM: Sumoylated on Lys-108; which negatively regulates transcriptional activity. Desumoylated specifically by SENP6. SIMILARITY: Belongs to the nuclear hormone receptor family. NR2 subfamily. & Contains 1 nuclear receptor DNA-binding domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |