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Millipore This Cyp7a1 Antibody Is Validated For Use In Wb & Ihc For The Detection Of The Cyp7a1 Protein. - Mill (Additional S&H or Hazmat Fees May Apply)
Cytochrome P450 7A1 (CYP7A1, CYPVII), also known as cholesterol 7-alpha-monooxygenase or cholesterol 7-alpha-hydroxylase, belongs to the cytochrome P450 family and is important for cholesterol homeostasis. CYP7A1 catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Detected primarily in the liver, CYP7A1 catalyzes the following reaction: Cholesterol + NADPH + O2 = 7-alpha-hydroxycholesterol + NADP+ + H2O. CYP7A1 is up-regulated by glucose and by cholestyramine, and is down-regulated by chenodeoxycholic acid.
Product Information
Format
Purified
Control
Human liver tissue lysate
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Cyp7a1 antibody is validated for use in WB & IHC for the detection of the Cyp7a1 protein.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Imunnohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Cyp7a1 in rat and human liver tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human Cyp7a1.
Clone
15B9.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Rat
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000771
Gene Symbol
CYP7A1
CYP7
Purification Method
Protein G Purified
UniProt Number
P22680
Molecular Weight
~52 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human liver tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cyp7a1 in 10 µg of human liver tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
