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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IHC, IP, RIA, Inhibition | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS461 |
| Description | Anti-Neutrophil Elastase Antibody, clone AHN-10 |
| Alternate Names |
|
| Background Information | Neutrophil Elastase, also known as Bone marrow serine protease, Elastase-2, Human leukocyte elastase (HLE), Medullasin or PMN elastase, and encoded by the gene ELANE/ELA2, is a serine proteinase in the same family as chymotrypsin. Neutrophil Elastase has broad substrate specificity and is part of our host defense mechanism against bacteria. Unfortunately passively released or actively secreted elastase from neutrophils has been linked to the pathologic processes of a variety of inflammatory diseases, including idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome, and cystic fibrosis. Neutrophil Elastase is localized to the cytoplasm in granules. Neutrophil Elastase is expressed in neutrophils and macrophages. Defects in Neutrophil Elastase may be associated with cyclic neutropenia and congential neutropenia diseases where neutrophils fail to mature leaving the patient very vunderable to infections. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Elastase using this mouse monoclonal antibody, Anti-Neutrophil Elastase Antibody, clone AHN-10 validated for use in ICC, IHC, IP, Radioimmunoassay & Inhibition. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in unirradiated and irradiated human and mouse skin tissues (Rhodes, L. E., et al. (2009). FASEB J. 23(11): 3947-3956.; Nicolaou, A., et al. (2012). Photochem Photobiol Sci. 11(2):371-380.; Rutter, J. J., et al. (2009). 129(12):2861-2867.). Western Blotting Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in human pancreatic tissue lysate (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.). Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Neutrophil Elastase in human pancreatic tissue lysate (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in human acinar cells (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.). Inhibition Analysis: This assay inhibits the elastinolytic actiity of purified human Neutrophil Elastase (Skubitz, K., et al. (1988). J Leukoc Biol. 44(3):158-165.). Radioimmunoassay Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase using purified Neutrophil Elastase (Skubitz, K., et al. (1988). J Leukoc Biol. 44(3):158-165.). |
| Biological Information | |
|---|---|
| Immunogen | Whole cells corresponding to human Neutrophil Elastase. |
| Clone | AHN-10 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody detects with human Neutrophil Elastase but not other major neutrophil proteases: Cathapsin G, proteinase 3, collagenase, or esterase N (Skubitz, K. M., et al. (1988). J Leukoc Biol. 44(3):158-165.). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | 28 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry Analysis in peripheral blood mononuclear cells. Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Neutrophil Elastase in peripheral blood mononuclear cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |