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Key Specifications Table
| Key Applications |
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| Cult |
| Description | |
|---|---|
| Catalogue Number | PMEF-CFL-P1 |
| Trade Name |
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| Description | EmbryoMax® Primary Mouse Embryonic Fibroblasts |
| Overview | PMEF-CFL-P1 are low passage Primary Mouse Embryo Fibroblasts (PMEFs) isolated from CF1 mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells. |
| Alternate Names |
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| Background Information | The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow. |
| Product Information | |
|---|---|
| Presentation | Provided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | PMEF, Strain CF1, Not Treated, Passage 1 |
| Key Applications |
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| Biological Information | |
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| Stem Cell Type |
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| Cell Line Type |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years. |
| Packaging Information | |
|---|---|
| Material Size | 1 vial |
| Material Package | 1x106 cells |