| Description | |
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| Catalogue Number | S8000 |
| Brand Family | Upstate |
| Trade Name |
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| Description | CpGenome™ Universal Methylated Mouse DNA Standard Set |
| Overview | The CpGenome™ Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice and enzymatically methylated at all CpGs by M.SssI methyltransferase. It can be used as a positive control for gene methylation studies, and is fully compatible with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847) and CpG WIZ® methylation-specific amplification systems. Materials Provided: One vial containing 5 µg (20 µl) of CpGenome™ Universal Methylated Mouse DNA Standard at a concentration of 250 ng/µL. Validation: Methylation-specific PCR (MSP) was performed on the CpGenome™ Universal Methylated Mouse DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ® Oct-4 kits (Catalog No. S7840) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for this Standard. CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146. |
| Product Information | |
|---|---|
| Presentation | Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 |
| Quality Level | MQ100 |
| Applications | |
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| Application | The CpGenome Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice & enzymatically methylated at all CpGs by M.SssI methyltransferase. |
| Application Notes | For MSP primer design, please use the MethPrime software package. Click here |
| Biological Information | |
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| Product Usage Statements | |
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| Storage and Shipping Information | |
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| Storage Conditions | Recommended Storage: Stable at -20°C for up to 6 months from date of receipt. |
| Packaging Information | |
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| Material Size | 5 µg |