Miltenyi Biotec Il-10 Antibody, Anti-Human, Pe

Miltenyi Biotec Interleukin 10 (IL‑10) is a cytokine predominantly secreted by CD4+ memory and effector T cells and antigen-presenting cells, for example, monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by Tʜ1 cells, activated monocytes, and NK cells. It can stimulate immunoglobulin production by B cells. | The Anti-IL‑10 antibodies has been designed for intracellular staining of IL‑10–producing cells. Cells can be stimulated for IL‑10 production, for example, by polyclonal stimulation with mitogens. For induction of IL‑10 production by antigen-specific T cells, cells are re-stimulated with the respective antigen. IL‑10 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL‑10–producing cells can be stained intracellularly with Anti-IL‑10 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL‑10–producing cells. | Magnetically enriched cells can be stained intracellularly for IL‑10 production directly on the MACS Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4+ T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids. - MLTY (Additional S&H or Hazmat Fees May Apply)