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Cell Signaling Dykddddk Tag (D6w5b) Rabbit mAb (Binds To Same Epitope As Sigmas Anti-Flag M2 Antibody)
Cell Signaling Dykddddk Tag (D6w5b) Rabbit mAb (Binds To Same Epitope As Sigmas Anti-Flag M2 Antibody) - CSIG (Additional S&H or Hazmat Fees May Apply)
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$327.00
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| NETA PART: | CSIG-14793S |
| MFG.PART: | 14793S |
| UNSPSC: | 41120000 |
| Manufacturer: | Cell Signaling |
| Size | 100 µl |
| Reactivity | All |
| Sensitivity | Transfected Only |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:400 - 1:1600, Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600, Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:3200, Chromatin IP: 1:50} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #70586. |
| Specificity/Sensitivity | DYKDDDDK Tag (D6W5B) Rabbit mAb detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigmas Anti-FLAG® antibodies, fused to either the amino-terminus or carboxy-terminus of the target protein. |
| Species Reactivity | All Species Expected |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.tThe DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1). |
| SKU | CSIG-14793S |
|---|---|
| Supplier Part Number | 14793S |
| UM | UNIT |
| UNSPSC | 41120000 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |
| Hazardous | N |