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Genscript GenCRISPR™ Cas13a (C2c2) Nuclease
Genscript GenCRISPR™ Cas13a (C2c2) Nuclease - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$189.00
Your Price
$189.00
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| NETA PART: | GSCRPT-Z03486-100 |
| MFG.PART: | Z03486-100 |
| UNSPSC: | 12352202 |
| Manufacturer: | GenScript USA Inc |
Overview
| Description | GenCRISPR™ Cas13a (C2c2) Nuclease is an RNA-guided RNA endonuclease from Leptotrichia wadei. This recombinant nuclease is purified from E. coli expression and contains C-terminal His Tag. Cas13a (previously known as C2c2) belongs to the Class 2 Type VI CRISPR-Cas system and contains a single protein effector with two HEPN domains. Cas13a exhibits two distinct ribonuclease activities. Cas13a possesses sequence-specific RNA cleavage activity in vitro and in vivo. And after specific cleavage, Cas13a activates collateral cleavage activity towards adjacent non-specific ssRNA sequences. The two distinct ribonuclease activities of Cas13a enable guide-RNA processing and RNA detection. The RNA detection empowered with Cas13a-crRNA complex and quenched fluorescent ssRNA as reporter has been reported to be versatile, rapid and high-specific. |
Applications
| crRNA-dependent RNA cleavage |
Properties
| Source | Recombinant Cas13a expressed by E.coli |
| Species | Leptotrichia wadei |
| Tag | C-terminal His Tag |
| Apparent Molecular Weight | ~140 kDa, on SDS-PAGE under non-reducing conditions |
| Concentration | Please refer to the COA for the specific lot. |
| Storage Buffer | 10 mM Tris-HCl, 300 mM NaCl, 1.0 mM DTT, 50% glycerol, pH 8.0. |
| Storage & Stability | Store at -20 °C for up to 12 months from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| Accession# | U2PWF1 |
Quality Control Specifications
| Appearance | Clear, colorless liquid |
| Purity | ≥ 95% by SDS-PAGE |
| Activity | ≥ 85% by in vitro digestion of ssRNA |
| Concentration | 4 mg/ml ± 10% as analyzed by A280 |
| Residual DNase | Undetectable |
| Residual RNase | Undetectable |
| Endotoxin Level | < 0.2 EU/μg |
Background
| References | 1. Abudayyeh, Omar O., et al. "C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector." Science 353.6299 (2016). |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| SKU | GSCRPT-Z03486-100 |
|---|---|
| Supplier Part Number | Z03486-100 |
| UM | UN |
| UNSPSC | 12352202 |
| Manufacturer | GenScript USA Inc |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 7 Business Days |
| Hazardous | N |