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GenScript Benz-Neburase™ GMP, His-tag10kU
GenScript Benz-Neburase™ GMP, His-tag10kU - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$58.50
Your Price
$58.50
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NETA PART: | GSCRPT-Z03627-10 |
MFG.PART: | Z03627-10 |
UNSPSC: | 12352204 |
Manufacturer: | GenScript USA Inc |
Overview
Product Introduction | Benz-Neburase is a genetically engineered endonuclease from Serratia marcescens used in the purification processes of biological molecules and biopharmaceutical production such as vaccine, viral vector, gene and cell therapy manufacturing facilities. The enzyme is highly effective, nonspecific endonuclease capable of digesting all forms of DNA and RNA into 3-5 bp nucleotides. Benz-Neburase is active in a broad range of conditions and is free of proteolytic activity, making the enzyme especially useful for applications with challenging DNA contaminants, such as lysed host cells in viral vector manufacturing processes. The nuclease requires divalent cation, preferably Mg2+ for activity, displays a broad pH tolerance, ranging from pH 6 to pH 10, optimal at 8-8.5, and has a wide temperature tolerance, ranging from 35 °C to 55 °C. Benz-Neburase is a physiologic homodimer and functions more progressively than the monomer. Two disulfide bonds in the nuclease are crucial to its activity and stability. GenScript’s offerings are cost-effective with reliable and proven performance for removal of contaminating nucleic acids. Furthermore, the Benz-Neburase’s offerings are produced under stringent quality controls with materials and manufacturing process that are fully traceable, allowing for support of IND filing and progression of development stages for various applications. |
Properties
Expression System | E.coli |
Species | Serratia Marcescen |
Tag | N-terminal 6 × His |
Theoretical Molecular Weight | 27.5 kDa |
Biological Activity | ≥ 1.1 × 106 U/mg One unit of Benz-Neburase™ GMP, His-tag is defined as the amount of enzyme that causes a ∆A260 of 1.0 (equivalent to the complete digestion of 37 μg DNA) in 30 min. |
Formulation | Supplied as a solution of 20 mM Tris-HCl, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol, pH 8.0. |
Storage & Stability | This product remains stable for up to 2 weeks at 4 °C or up to 30 months at -20 °C. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
Applications
This enzyme requires 1-2 mM Mg2+ to be used to remove all forms of DNA and RNA, including double stranded, single stranded, linearized, and circular forms. |
Quality Control Specifications
Appearance | Clear, colorless liquid |
Purity | ≥ 99% as analyzed by SEC-HPLC ≥ 95% as analyzed by SDS-PAGE |
Enzyme Activity | ≥ 250 U/μl |
Endotoxin Level | ≤ 0.01 EU/kU |
Residual HCP | ≤ 10 µg/mg |
Protease Activity | Non-detectable |
Bioburden | < 1 CFU/ml |
Heavy Metal Residue | ≤ 10 ppm |
Mycoplasma | Negative |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
SKU | GSCRPT-Z03627-10 |
---|---|
Supplier Part Number | Z03627-10 |
UM | EA |
UNSPSC | 12352204 |
Manufacturer | GenScript USA Inc |
ProductLine | GSCRPT |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 7 Business Days |
Hazardous | N |