Takara Pfu N-acetyl Deblocking Aminopeptidase (Ac-DAP) \50 ug

Takara Pfu N-acetyl Deblocking Aminopeptidase (Ac-DAP) is a unique exo-type aminopeptidase that liberates blocking groups (formyl, acetyl, and myristyl), and then releases the subsequent amino acids from proteins and peptides until it reaches the first X-Pro bond. This enzyme has a wide range of substrate specificities and is used to determine short stretches of amino acid sequence of blocked proteins and peptides whose sequence can be determined by Mass Spectrometry. In addition, since the amino terminus of Ac-DAP is acetylated, its amino acid sequence cannot be determined by Edman degradation. Therefore, even if a high E/S ratio is used (for example, E/S = 0.5-1), the amino acid sequence of the target protein or peptide can still be determined by Edman Degradation without requiring separation from the enzyme. - TAKBGN (Additional S&H or Hazmat Fees May Apply)