We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Share this :
Print
Cell Signaling Cdk2 (E8j9t) Xp Rabbit mAb
Cell Signaling Cdk2 (E8j9t) Xp Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$165.00
Your Price
$165.00
HOW MUCH YOU SAVE:
0.00 %
NETA PART: | CSIG-18048T |
MFG.PART: | 18048T |
UNSPSC: | 12352203 |
Manufacturer: | Cell Signaling |
Size | 20 µl |
Reactivity | H M R |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 33 |
Source/Isotype | Rabbit IgG |
Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunohistochemistry (Paraffin): 1:200 - 1:800, Immunofluorescence (Frozen): 1:400 - 1:1600, Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #34541. |
Specificity/Sensitivity | CDK2 (E8J9T) XP® Rabbit mAb recognizes endogenous levels of total CDK2 protein. In rodent cell lines, isoforms 1 and 2 of CDK2 run as two distinct bands by western blot. This antibody non-specifically labels the muscle in the mouse retina and the vasculature in the mouse brain by immunofluorescence. |
Species Reactivity | Human, Mouse, Rat |
Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro294 of human CDK2 protein. |
Background | Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5). |
SKU | CSIG-18048T |
---|---|
Supplier Part Number | 18048T |
UM | EA |
UNSPSC | 12352203 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |
Hazardous | N |