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Cell Signaling Phospho-(Ser) Arg-X-Tyr/Phe-X-Pser Motif Antibody
Cell Signaling Phospho-(Ser) Arg-X-Tyr/Phe-X-Pser Motif Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
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$394.00
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$394.00
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NETA PART: | CSIG-2981S |
MFG.PART: | 2981S |
UNSPSC: | 12352203 |
Manufacturer: | Cell Signaling |
Size | 100 µl |
Reactivity | All |
Sensitivity | Endogenous |
Source/Isotype | Rabbit |
Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Peptide ELISA (DELFIA): 1:1000} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody detects endogenous levels of proteins containing the Arg-X-Tyr/Phe-X-pSer motif. This antibody does not cross-react with nonphosphorylated serine or phospho-threonine proteins with the same motif or other phospho-serine/threonine-containing proteins and peptides without this motif. |
Species Reactivity | All Species Expected |
Source/Purification | Polyclonal antibodies are produced by immunizing animals with Arg-X-Tyr/Phe-X-pSer-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography. |
Background | The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Binding of 14-3-3 is mediated through phospho-serine-containing proteins (6). Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (6). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCgamma, IRS-1 and BCR (6). Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif. |
SKU | CSIG-2981S |
---|---|
Supplier Part Number | 2981S |
UM | EA |
UNSPSC | 12352203 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |
Hazardous | N |