We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Share this :
Print
Cell Signaling Cleaved Rock1 (Gly1114) Antibody
Cell Signaling Cleaved Rock1 (Gly1114) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$353.00
Your Price
$353.00
HOW MUCH YOU SAVE:
0.00 %
| NETA PART: | CSIG-71001S |
| MFG.PART: | 71001S |
| UNSPSC: | 12352203 |
| Manufacturer: | Cell Signaling |
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 29 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Cleaved ROCK1 (Gly1114) Antibody recognizes endogenous levels of ROCK1 protein only when cleaved at Gly1114. This antibody detects the carboxy-terminal cleavage product. In HeLa cells, this antibody cross-reacts with a 60 kDa band of unknown origin by western blot. |
| Species Reactivity | Human |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1114 of human ROCK1 protein. Antibodies are purified by peptide affinity chromatography. |
| Background | ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence resulting in loss of its C-terminal inhibitory domain (3). ROCK2 is directly cleaved by granzyme B (grB). Cleavage activates ROCK and leads to phosphorylation of myosin light chain (MLC) and inhibition of myosin phosphatase (4). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (5-7). |
| SKU | CSIG-71001S |
|---|---|
| Supplier Part Number | 71001S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| CountryOfOrigin | US |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |
| Hazardous | N |