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GenScript MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit specifically recognizes His tags placed at C-terminal and internal regions of His-fusion proteins. This product is not recommended for N-terminal His-fusion protein detection.
Host Species
Rabbit
Immunogen
6x His synthetic peptide coupled to KLH
Conjugate
Unconjugated
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application
Recommended Usage
ELISA
0.05-0.2 μg/ml
Western Blot
0.1-0.2 μg/ml
Immunoprecipitation (IP)
2 μg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF)
1 μg/ml
Flow Cytometry
1 μg/ml
Properties
Form
Lyophilized
Storage Buffer
Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Storage Instructions
The lyophilized product remains stable for up to 1 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C, or for up to 12 months at -20°C or below. Upon receiving, spin the vial down to place the lyophilized antibody on the bottom of the vial. Reconstitute the antibody as mentioned above. Prepare single use aliquots (no less than 10µl) and freeze it immediately at -20oC. The frozen aliquots should be thawed once with any remainder kept at 4oC.
Purification
Purified by Protein A affinity chromatograph.
Isotype
Rabbit IgG
Clonality
Monoclonal
Clone ID
25B6E11
Note
GenScript can customize this product according to you requirement, including product size, buffer components, etc.
Background
Target Background
The rabbit immune system generates diverse antibodies and optimizes antibody affinity. GenScript utilizes MonoRabTM technology to generate high affinity and specific monoclonal rabbit antibodies. GenScript’s MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit is specific to six histidine tag placed at the C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins from bacteria, insect cells, and mammalian cells.
Synonyms
Rabbit monoclonal to His tag
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
