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| Description | |
|---|---|
| Catalogue Number | 04-1557-25UL |
| Description | Anti-SIRT1 Antibody, clone 10E4 (mouse monoclonal) |
| Alternate Names |
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| Background Information | Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organisms response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-SIRT1 Antibody, clone 10E4 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC, ChIP to detect SIRT1 also known as sirtuin (silent mating type information regulation 2 homolog) 1, sirtuin 1. |
| Key Applications |
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| Application Notes | Immunoprecipitation: Reported by independent researcher using representative lot. Chromatin Immunopreiciptiation: Reported by independent researcher using representative lot. Immunohistochemistry: Reported by independent researcher using representative lot. |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant protein corresponding to human SIRT1. |
| Epitope | Unknown |
| Clone | 10-E-4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | This antibody recognizes SIRT1. |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with human. Does not react in mouse in IHC. Mouse sequence is 100% identical to immunogen, so it is predicted to work in western blotting. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates Lys-382 of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2- mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. COFACTOR: Binds 1 zinc ion per subunit (By similarity). SIZE: 747 amino acids; 81681 Da SUBUNIT: Interacts with TAF1B. Found in a complex with PCAF and MYOD1 (By similarity). Interacts with MLLT7/FOXO4, HES1, HEY2, p53/TP53 and PML. SUBCELLULAR LOCATION: Nucleus. Note=Recruited to the nuclear bodies via its interaction with PML.TISSUE SPECIFICITY: Widely expressed. SIMILARITY: Belongs to the sirtuin family. & Contains 1 deacetylase sirtuin-type domain. MISCELLANEOUS: Red wine, which contains resveratrol, may participate in activation of sirtuin proteins, and may therefore participate in an extended lifespan as it has been observed in yeast. |
| Molecular Weight | ~ 110 kDa |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/ml of this antibody detected SIRT1 on 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 µL |