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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ELISA, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1135-25UG |
| Description | Anti-PDGFR-b Antibody, clone CH-3 (mouse monoclonal) |
| Alternate Names |
|
| Background Information | PDGFR (Platelet-Derived Growth Factor Receptor) is a receptor tyrosine kinase for the PDGF family of growth factors. PDGF homo and hetero- dimmers (dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB)) bind to the two structurally related a and b PDGF Receptors (PDGFRa and PDGFRb). The type A PDGF Receptor binds all three PDGFs with high affinity, whereas the type B PDGF Receptor is selective for PDGF-BB. This binding results in the dimerization of the receptor and the subsequent kinase activation of the receptor tyrosine kinase (RTK) and its autophosphorylation. These phosphorylations allow for various proteins, such as PI3 Kinase, PLCg, and Grb2, to bind through their SH2 domains and transduce signals into the cell. The activation of PDGFFR triggers the intracellular loop and activates Ras, Erk1/2, and ROS. PDGFb has been implicated in embryonic development, angiogenesis, and development of tumors of glial origin. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 in buffer containing 20 mM sodium phosphate, 250 mM NaCl, 0.1% NaN3, pH. 7.6 |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-PDGFR Antibody, clone CH-3 is validated for use in WB, ELISA, IH(P) for the detection of PDGFR. |
| Key Applications |
|
| Application Notes | ELISA: A previous lot of this antibody was used in ELISA. Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of PDGF-A With Citrate pH 6.0 Epitope Retrieval: Rat Brain |
| Biological Information | |
|---|---|
| Immunogen | Recombinant Platelet-Derived Growth Factor Receptor |
| Clone | CH-3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human and mouse. Expected to react with rat based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| Molecular Weight | 211 amino acids; 24043 Da |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Jurkat lysates. Western Blot Analysis: 1:500 dilution of this lot detected PDGFR on 10 μg of Jurkat lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at + 2-8 ° C in undiluted aliquots for up to 1 year from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |