Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, ICC, ChIP | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1863-25UG |
| Description | Anti-PAGR1 (PA1) |
| Alternate Names |
|
| Background Information | PAXIP1-associated glutamate-rich protein 1 (UniProt: Q9BTK6; also known as Glutamate-rich co-activator interacting with SRC11, GAS1, PAXIP1-associated protein 1, PTIP-associated protein 1) is encoded by the PAGR1 (also known as C16orf53, PA1) gene (Gene ID: 79447) in human. It associates with the histone methyltransferase MLL2/MLL3 complex, which suggests that it plays a role in epigenetic transcriptional activation. However, in association with PAXIP1/PTIP is proposed to function at least in part independently of the MLL2/MLL3 complex. It is proposed to be recruited by PAXIP1 to sites of DNA damage where the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage independently of the MLL2/MLL3 complex. During immunoglobulin class switching in activated B-cells, it is involved in transcription regulation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus independently of the MLL2/MLL3 complex. PAGR1 protein is reported to act as a transcriptional cofactor for nuclear hormone receptors. It also inhibits the induction properties of several steroid receptors such as NR3C1, AR and PPARG; the mechanism of inhibition appears to be gene-dependent. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-PAGR1 (PA1), Cat. No. ABE1863-25UG, is a highly specific rabbit polyclonal antibody, that targets PAXIP1-associated glutamate-rich protein 1 and has been tested in Western Blotting, Immunocytochemistry and Chromatin Immunoprecipitation (ChIP). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected PAGR1 (PA1) in Western Blotting applications (Lee, J.E., et. al. (2013). Elife. 2:e01503; Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93). Chromatin Immunoprecipitation Analysis: A representative lot detected PAGR1 (PA1) in MEFs (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39). Immunocytochemistry Analysis: A representative lot detected PAGR1 (PA1) in U2OS & HeLa cells (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full-length human recombinant PAXIP1-associated protein 1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects PAXIP1-associated glutamate-rich protein 1 in human and mouse. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | 42 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell nuclear extract. Western Blotting Analysis: 0.5 µg/mL of this antibody detected PAGR1 (PA1) in 10 µg of HEK293 cell nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |