Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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H | WB, IP | Rb | Purified | Polyclonal Antibody |
Description | |
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Catalogue Number | ABE2873-25UL |
Description | Anti-NUDT21 |
Alternate Names |
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Background Information | Cleavage and polyadenylation specificity factor subunit 5 (UniProt: O43809; also known as Cleavage and polyadenylation specificity factor 25 kDa subunit, CFIm25, CPSF 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 25 kDa subunit) is encoded by the NUDT21 (also known as CFIM25, CPSF25, CPSF5) gene (Gene ID: 11051) in human. CFlm25 is a homodimeric protein that is a component of the cleavage factor Im (CFIm) complex, which plays a key role in pre-mRNA 3-processing. It is involved in association with CPSF6 or CPSF7 in pre-MRNA 3-end poly(A) site cleavage and poly(A) addition. The interaction of CFlm25 with the RNA is one of the earliest steps in the assembly of the 3 end processing complex and facilitates the recruitment of other processing factors. It contains one Nudix hydrolase domain (aa 76-201). The region containing aa 2-147 is considered to be essential for RNA-binding. It can be acetylated by p300/CBP and acetylation results in a reduction in its interaction with Poly(A) polymerase alpha (PAPAO). |
Product Information | |
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Format | Purified |
Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
Applications | |
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Application | Anti-NUDT21, Cat. No. ABE2873, is a highly specific rabbit polyclonal antibody, that targets Cleavage and polyadenylation specificity factor subunit 5 and has been tested in Immunoprecipitation and Western Blotting. |
Key Applications |
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Application Notes | Western Blotting Analysis: A representative lot detected NUDT21 in RNase A-treated HeLa whole-cell extract after IP (Katahira, J., et. al. (2013). Nucleic Acid Res. .41(14):7060-72). Immunoprecipitation Analysis: A representative lot detected NUDT21 in Co-immunoprecipitation with FLAG-tagged CF I cell lysates (Shimazu, T., et. al. (2007). J Biol Chem. 282(7):4470-8). |
Biological Information | |
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Immunogen | His-tagged full length recombinant human Cleavage and polyadenylation specificity factor subunit 5 (CFIm25). |
Concentration | Please refer to lot specific datasheet. |
Host | Rabbit |
Specificity | This rabbit polyclonal antibody detects Cleavage and polyadenylation specificity factor subunit 5 (CFIm25) in human cells. |
Isotype | IgG |
Species Reactivity |
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Species Reactivity Note | Human. |
Antibody Type | Polyclonal Antibody |
Gene Symbol |
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Purification Method | Affinity Purified |
UniProt Number |
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Molecular Weight | 25 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
Product Usage Statements | |
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Quality Assurance | Evaluated by Western Blotting in Caco-2 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected NUDT21 in 10 µg of Caco-2 cell lysate. |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
Packaging Information | |
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Material Size | 25 µL |