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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, DB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE411-S |
| Description | Anti-dimethyl Histone H3 (Arg26), Asymmetric, Trial Size Antibody |
| Alternate Names |
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| Background Information | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-dimethyl Histone H3 (Arg26) antibody, Asymmetric, Trial Size is validated for use in western blotting, ICC & dot blot for the detection of dimethyl Histone H3 (Arg26). |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected dimethyl Histone H3 (Arg26), Asymmetric in A431 and HepG2 cells. Dot Blot (Specificity) Analysis: 0.5 µg/mL from a representative lot detected dimethyl Histone H3 (Arg26), Asymmetric in Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665). DyLight® is a registered trademark of Thermo Fisher Scientific. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3, asymmetrically dimethylated at Arg26. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Human. Broad species reactivity is expected based on immunogen sequence similarities. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in untreated and CARM1 treated HeLa acid extracts. Western Blotting Analysis: 1 µg/mL of this antibody detects dimethyl Histone H3 (Arg26), Asymmetric in 10 µg of CARM1 treated HeLa acid extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |