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| Description | |
|---|---|
| Catalogue Number | AC146 |
| Trade Name |
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| Description | Normal Chicken IgY Antibody, purified | AC146 |
| Background Information | Chickens transfer high quantities of IgY (IgG) into the egg yolk and harvesting antibodies from eggs eliminates the need for the invasive bleeding procedure. One weeks eggs can contain 10 times more antibodies than the volume of rabbit blood obtained from one weekly bleeding. However, there are some disadvantages when using certain chicken derived antibodies in immunoassays. Chicken IgY does not fix mammalian complement component C1 and it does not perform as a precipitating antibody using standard solutions. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified chicken polyclonal in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Application Notes | Gel Electrophoresis Analysis: 20 µg of reduced Chicken IgY from a representative lot was resolved by SDS-PAGE and visualized using total protein stain. Note: To be used as a negative control for ChIP Ab+ MeCP2, Cat. No. 17-10491. Note: For use as a negative control, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. |
| Biological Information | |
|---|---|
| Concentration | 1 mg/mL |
| Host | Chicken |
| Isotype | IgY |
| Antibody Type | Polyclonal Antibody |
| Purification Method | T-gel |
| Molecular Weight | ~180 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting using non-reduced Chicken IgY. Western Blotting Analysis: 0.01 µg of this non-reduced Chicken IgY was detected by a Rabbit anti-Chicken secondary antibody. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |