Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB430-I-100UG |
| Description | Anti-Epithelium Endothelial Cells Antibody, clone PHM-5 |
| Alternate Names |
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| Background Information | The glomeruli network of capillaries filters the blood and the filtrate then enters the renal tubule. Unlike most capillary beds, the glomerular capillaries exit into efferent arterioles rather than venules. The resistance that exists in efferent arterioles causes sufficient hydrostatic pressure within the glomerulus to provide the force for ultrafiltration. Glomerular endothelial cells that create an interface between blood and tissue compartments are responsible for regulating vasomotor tone, trafficking of leukocytes, and maintenance of maintain hemostasis. Unlike endothelium in other tissues, the normal glomerular endothelium is extremely attenuated, being only about 50 to 150 nm in height around the perimeter of the capillary loops. Glomeruli of human kidneys are shown to be rich sialoglycoprotein, which is chemically distinct from rat podocalyxin. (Ref.: Ballermann, BJ (2005). Kidney Int. 67(5); 1668-1671; Kerjaschki, D., et al. (1986). J. Clin. Invest. 78(5); 1142-1149). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Epithelium Endothelial Cells, clone PHM-5, Cat. No. MAB430-I, is a mouse monoclonal antibody that detects human human epithelium/endothelial cells and has been tested for use in Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Epithelium Endothelial Cells in human cerebral cortex tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | Human Glomerular cells. |
| Clone | 18.29 (PHM-5) |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone PHM-5 is a mouse monoclonal antibody that detects human epithelium/endothelial cells. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Purification Method | Protein G purified |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Epithelium Endothelial Cells in human kidney tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |