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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE159 |
| Description | Anti-Fox1 Antibody, clone D8F8 |
| Alternate Names |
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| Background Information | Fox1, also commonly referred to as Ataxin-2-binding protein 1, is an RNA binding protein involved in the regulation of tissue-specific alternative splicing. This protein contains a highly conserved RRM (RNA recognition motif) and associates with 5-UGCAUGU-3 elements. This protein has been demonstrated to regulate alternative splicing of tissue specific exons and differentially spliced exons during erythropoiesis. Its alternative name, Ataxin-2-binding protein, is a result of its association with the C terminus of ataxin 2 which may contribute to the pathology of the neurogenerative disease spinocerebellar ataxia type 2 (SCA2). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Fox1 Antibody, clone D8F8 is a Mouse Monoclonal Antibody for detection of Fox1 also known as hexaribonucleotide binding protein 1, Ataxin-2-binding protein 1 & has been validated in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to mouse Fox1. |
| Clone | D8F8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G |
| UniProt Number |
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| UniProt Summary | FUNCTION: RNA-binding protein that regulates alternative splicing events by binding to 5-UGCAUGU-3 elements. Prevents binding of U2AF2 to the 3-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis. SUBUNIT STRUCTURE: Binds to the C-terminal of ATXN2. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Predominantly nuclear. TISSUE SPECIFICTY: Detected in brain (at protein level). Detected in heart, brain, neurons, skeletal muscle and embryo. SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain. |
| Molecular Weight | ~55 kDa observed. Uncharacterized bands potentially due to alternative splicing have been observed at ~33 and 37 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse heart tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Fox1 on 10 µg of mouse heart tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |