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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, IF, FC | M | PE | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF522 |
| Description | Anti-CD11c Antibody (human), PE, clone 3.9 |
| Alternate Names |
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| Background Information | The 3.9 antibody reacts with human CD11c, also known as integrin alpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha X (CD11c) assembles with Integrin beta-2 (CD18) into a receptor known as CR4 which can bind and induce signaling throµgh ICAMs and VCAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The 3.9 antibody is widely used as a marker for CD11c expression on dendritic cells (DC), often in parallel with markers for CD11b, for identification of developmental stages and mature subsets of this cell type. CD11c is prominently expressed on tissue macrophages, and is also detected on activated neutrophils, granulocytes, some types of activated T cells and intestinal intraepithelial lymphocytes (IEL). The antibody is reported to be cross-reactive with Baboon, Chimpanzee, Cynomolgus and Rhesus CD11c. |
| Product Information | |
|---|---|
| Format | PE |
| Presentation | Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal antibody Anti-CD11c (human), PE, clone 3.9 validated for use in IP, Immunofluorescence & Flow Cytometry for the detection of CD11c. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Immunogen information for this antibody is proprietary information. |
| Clone | 3.9 |
| Concentration | 5 µL (1 µg)/test |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Size Exclusion Chromatography |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Flow Cytometry Analysis: 1 µg of this antibody detected CD11c in one million human peripheral blood monocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Packaging Information | |
|---|---|
| Material Size | 25 tests |