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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IP, IHC, FC | R | PerCP-Cy5.5 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF550 |
| Description | Anti-CD25 Antibody (mouse), PerCP-Cy5.5, clone PC61.5 |
| Alternate Names |
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| Background Information | The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-. |
| Product Information | |
|---|---|
| Format | PerCP-Cy5.5 |
| Presentation | Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rat monoclonal antibody Anti-CD25 (mouse), PerCP-Cy5.5, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Immunogen information for this antibody is proprietary information. |
| Clone | PC61.5 |
| Concentration | 0.2 mg/mL |
| Host | Rat |
| Isotype | IgG1λ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Size Exclusion Chromatography |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Flow Cytometry Analysis: 0.25 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Packaging Information | |
|---|---|
| Material Size | 25 µg |