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Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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H | FC | M | APC-Cy7 | Monoclonal Antibody |
Description | |
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Catalogue Number | MABF552 |
Description | Anti-CD27 Antibody (human), APC-Cy7, clone O323 |
Alternate Names |
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Background Information | The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells.The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naïve vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD27, CD1d, IL-10 and TGF-beta (Kessel et al. 2012. Autoimm. Rev. 11(9): 670-677). The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27. |
Product Information | |
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Format | APC-Cy7 |
Presentation | Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide. |
Quality Level | MQ100 |
Applications | |
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Application | This mouse monoclonal antibody Anti-CD27 (human), APC-Cy7 & clone O323 validated for use in Flow Cytometry for the detection of CD27. |
Key Applications |
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Biological Information | |
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Immunogen | Immunogen information for this antibody is proprietary information. |
Clone | O323 |
Concentration | 5 μL (0.125 μg)/test |
Host | Mouse |
Isotype | IgG1κ |
Species Reactivity |
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Antibody Type | Monoclonal Antibody |
Gene Symbol |
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Purification Method | Size Exclusion Chromatography |
UniProt Number |
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Product Usage Statements | |
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Quality Assurance | The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Flow Cytometry Analysis: 0.125 μg of this antibody detected CD27 in one million human peripheral blood lymphocytes. |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
Packaging Information | |
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Material Size | 25 tests |