Special Offers
100% Performance Guaranteed
Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
---|---|---|---|---|
M | IP, IHC, FC | R | violetFluor™ 450 | Monoclonal Antibody |
Description | |
---|---|
Catalogue Number | MABF567 |
Trade Name |
|
Description | Anti-CD4 Antibody (mouse), violetFluor™ 450, clone GK1.5 |
Alternate Names |
|
Background Information | The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will “compete” for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4. |
Product Information | |
---|---|
Format | violetFluor™ 450 |
Presentation | Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide. |
Quality Level | MQ100 |
Applications | |
---|---|
Application | This rat monoclonal antibody Anti-CD4 (mouse), violetFluor 450, clone GK1.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD4. |
Key Applications |
|
Biological Information | |
---|---|
Immunogen | Immunogen information for this antibody is proprietary information. |
Clone | GK1.5 |
Concentration | 0.2 mg/mL |
Host | Rat |
Isotype | IgG2bκ |
Species Reactivity |
|
Antibody Type | Monoclonal Antibody |
Gene Symbol |
|
Purification Method | Size Exclusion Chromatography |
UniProt Number |
|
Product Usage Statements | |
---|---|
Quality Assurance | The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Flow Cytometry Analysis: 0.25 µg of this antibody detected CD4 in one million C57Bl/6 splenocytes. |
Usage Statement |
|
Storage and Shipping Information | |
---|---|
Storage Conditions | It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
Packaging Information | |
---|---|
Material Size | 25 µg |